Recently introduced PacBio Sequel/Sequel2 platforms, which rely on Single-Molecule Real Time (SMRT) sequencing technology, are one of the most widely used long-read sequencing approaches. ![]() These new sequencing platforms are undergoing active development and pushing boundaries in terms of total output, read length, sequencing time, cost reduction and read accuracy. The primary contenders in third-generation sequencing are Pacific Biosciences (PacBio) (Sequel, Sequel2) and Oxford Nanopore (MinION, GridION, and PromethION). The third-generation of sequencing is here and making tremendous impact in the field of genomics. The Creative Commons Public Domain Dedication waiver ( ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. ![]() Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
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